The plates represent a cross section of plates for different types of assays, cell growth and storage. We offer several formats: 96 and 24 wells to suit your needs.
Protocol for the screening of mammalian and insect cells – 96-well plates:
Material:
96-well plate, 2 ml, square wells, round bottom, individually wrapped with lid, sterile: 931134
96-well plate, 2 ml, square wells, pyramid bottom, individually wrapped with lid, sterile: 931133
Method:
- Maintain cell stocks in an appropriate growth medium. Divide cultures the day before transfection at an appropriate density to ensure growth of the log phase at the time of transfection.
- Add 500 µl / well of cell suspension. Optimal seed density depends on the cell line, please use the recommended density for the cell line used.
- Transfect the cells according to the established transfection protocol. Establish the ratio Transfection reagent / DNA / nutrients from what is used for the larger scale cultures.
- Seal the plates with plastic lids or gaskets and transfer them to a shaker overnight at 800 rpm and 37 ° C.
- Harvest cells at set time for larger scale cultures. Pellet the cells by centrifugation at 2,500 xg for 20 min at 4 ° C.
- Reserve culture medium or pellet depending on application and process downstream.
Notes:
The most critical factor in cell viability is aeration. Optimal results will be obtained using agitators with orbit diameters of 3 mm. We do not recommend working in 96-well plates using stirrers with standard diameters of 25 mm.
Thomson filter plates are an excellent add-on product for downstream purification applications.
96-well filter plate, 2 ml | 25µm Polypropylene: ref. 931919
Suggested maximum centrifugation: 3000 g.
Protocol for the screening of mammalian and insect cells – 24-well plates:
Material:
24-well plate, 10.4 mL, square wells, round bottom, individually wrapped with lid, sterile: 931568
24-well plate, 10.8 ml, square wells, pyramid bottom, individually wrapped with lid, sterile: 931571
Method:
- Maintain cell stocks in an appropriate growth medium. Divide cultures the day before transfection at an appropriate density to ensure growth of the log phase at the time of transfection.
- Place 4-5 ml / well of cell suspension. Optimal seed density depends on the cell line, please use the recommended density for the cell line used.
- Transfect the cells according to the established transfection protocol. Establish the ratio Transfection reagent / DNA / nutrients from what is used for the larger scale cultures.
- Seal the plates with plastic lids or gaskets and transfer them to a shaker overnight at 800 rpm and 37 ° C.
- Harvest cells at set time for larger scale cultures. Pellet the cells by centrifugation at 2,500 xg for 20 min at 4 ° C.
- Reserve culture medium or pellet depending on application and process downstream.
Notes:
The most critical factor in cell viability is aeration. Optimal results will be obtained using agitators with orbit diameters of 3 mm. We do not recommend working in 96-well plates using stirrers with standard diameters of 25 mm.
Thomson filter plates are an excellent add-on product for downstream purification applications.